Separation of Nucleotides by Thin-layer Chromatography

Separation of arabinosyl, ribosyl, and deoxyribosyl purine nucleotides by thin-layer chromatography.

Studies on the cellular metabolism of endogenous nucleosides and structurally related analogs are facilitated by the thin-layer chromatographic (TLC) separation of nucleotides, Complex mixtures of ribosyl and deoxyribosyl nucleotides can be separated by ascending two-dimensional TLC on PEI-cellulose anion-exchange layers”“. Nucleoside mono-, diand triphosphates are first separated in one dimens...

Separation of Cholesterol and Demosterol by Thin-layer Chromatography.

Strategies of sunscreen separation by thin layer chromatography.

So far sunscreens separation has been achieved primarily by HPLC or TLC on RP-18 or (less frequently) unmodified silica gel as stationary phase. The conditions of chromatographic separation published so far are not suitable for many combinations of UV filters found commonly in cosmetic products. It is therefore reasonable to seek alternative conditions that would make it possible to separate th...

Separation of plasma lipids by thin-layer chromatography.

A rapid comprehensive thin-layer chromatographic separation of the major plasma lipid classes on commercial silicic acid glass fiber paper is described. For screening purposes, oxidized fractions are rapidly visualized for qualitative interpretation. Standard colorimetric procedures for each lipid class are per­ formed upon the migration areas cut from the strips. This provides quantitative mea...

Complete analysis of cellular nucleotides by two-dimensional thin layer chromatography.

We describe methods for the complete analysis of cellular nucleotides from as few as 10(6) 32Pi-labeled cells in a simple 2-day experiment. Nucleotides are extracted with acid, neutralized, and resolved by two-dimensional thin layer chromatography on polyethyleneimine cellulose. In the first dimension the nucleotides are separated based on the negative charge of their phosphate groups (i.e. cyc...